Everything on bioinformatics, the science of information technology as applied to biological research.
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- Joined: Tue Apr 20, 2010 10:22 pm
Does anybody know how three different type ii restriction endonucleases can cut at the same sequence but at different points to yield three different set of products?
- Inland Taipan
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- Joined: Mon Sep 14, 2009 7:12 pm
That's pretty simple. The ER can produce either blunt ends or sticky ends with either 5'- or 3'-overhang. Thus if you had sequence GAATTC you can have:
Cis or trans? That's what matters.
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