Type ii restriction endonucleases

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Soni
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Type ii restriction endonucleases

Post by Soni » Tue Apr 20, 2010 10:30 pm

Does anybody know how three different type ii restriction endonucleases can cut at the same sequence but at different points to yield three different set of products?

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JackBean
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Post by JackBean » Wed Apr 21, 2010 9:22 am

That's pretty simple. The ER can produce either blunt ends or sticky ends with either 5'- or 3'-overhang. Thus if you had sequence GAATTC you can have:

G...AATTC
CTTAA...G

GAA...TTC
CTT...AAG

GAATT...C
C...TTAAG
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

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