Genetics as it applies to evolution, molecular biology, and medical aspects.

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Post by Anonymous1 » Fri Apr 16, 2010 12:06 am

I am confused about DNA polymerase in PCR :(

Maybe if someone could give me a brief explanation so I could hear it in different words?

What kind of DNA polymerase is used for PCR and how did the bacterium evolve to make such a useful polymerase? Specifically, why do we use the polymerase for PCR...? What are selective factors, and evolutionary fitness.

Thank you!

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Inland Taipan
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Post by JackBean » Fri Apr 16, 2010 7:42 am

DUring DNA replications there are many proteins, some of them are unwinding the DNA. But you don't want to put many proteins in the tube (it would be expensive and yet didn't work properly), so you're using temperature denaturation instead. So, you have to pick some polymerase, which could survive in like 97°C. I will guarantee you, that the human Pol would be dead, before you reached like 70°C. So, what organism you must to take ti from?

You could find a little advice here

Cis or trans? That's what matters.

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