Cetylpyridinium chloride solution storage

Discussion of all aspects of cellular structure, physiology and communication.

Moderators: Leonid, amiradm, BioTeam

Post Reply
jasmina
Garter
Garter
Posts: 37
Joined: Fri Sep 12, 2008 1:54 am
Contact:

Cetylpyridinium chloride solution storage

Post by jasmina » Tue Apr 13, 2010 11:07 am

hi there!
In my experiments, i used Cetylpyridinium chloride to treat my cells, i bought it from sigma http://www.sigmaaldrich.com/catalog/Pro ... KEY&F=SPEC, and dissolved it in autoclaved DDW, but i am not sure about its storage conditions, now i stored solution at 4 degree, i am wondering how long can it keep stable at 4 degree?

User avatar
MrMistery
Inland Taipan
Inland Taipan
Posts: 6832
Joined: Thu Mar 03, 2005 10:18 pm
Location: Romania(small and unimportant country)
Contact:

Post by MrMistery » Tue Apr 13, 2010 7:08 pm

if it was sterile, then it should probably be fine. Just try it and see if it works
"As a biologist, I firmly believe that when you're dead, you're dead. Except for what you live behind in history. That's the only afterlife" - J. Craig Venter

jasmina
Garter
Garter
Posts: 37
Joined: Fri Sep 12, 2008 1:54 am
Contact:

Re:

Post by jasmina » Wed Apr 14, 2010 1:21 am

MrMistery wrote:if it was sterile, then it should probably be fine. Just try it and see if it works


yes, i filter it with 0.2um filter, and now it works. i want to know can i store this solution at 4 degree for a long time ( for example, several months :) ) without any change ( concentration, degradation ).
:? :?

User avatar
MrMistery
Inland Taipan
Inland Taipan
Posts: 6832
Joined: Thu Mar 03, 2005 10:18 pm
Location: Romania(small and unimportant country)
Contact:

Post by MrMistery » Wed Apr 14, 2010 2:49 am

well pyridine is degraded by bacteria, that's why i said that you should keep it sterile. But i don't think it would chemically degrade in water (you have it in an aqueous solution right?). though myself i don't like to keep solutions for longer than a few months (that does happen, I sometimes keep growth media for more than a year, when I can see it turn yellow...)
"As a biologist, I firmly believe that when you're dead, you're dead. Except for what you live behind in history. That's the only afterlife" - J. Craig Venter

jasmina
Garter
Garter
Posts: 37
Joined: Fri Sep 12, 2008 1:54 am
Contact:

Post by jasmina » Wed Apr 14, 2010 5:21 am

thank you !^_^

Post Reply

Who is online

Users browsing this forum: No registered users and 0 guests