PCR no bands no primer

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jasmina
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PCR no bands no primer

Post by jasmina » Tue Apr 06, 2010 8:18 am

hi there!
When i do PCR, after electrophoresis, I did not get any bands, and there are no primer, i am wondering about this, if primer is used, why there is no amplification bands, if not, why i could not see primer bands?

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JackBean
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Post by JackBean » Tue Apr 06, 2010 8:44 am

the are so small, that they can easily run out of your gel, that's not much saying. EtBr binds to dsDNA, so if your primers do not form dimers, they probably won't bind EtBr much efficiently
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MrMistery
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Post by MrMistery » Tue Apr 06, 2010 8:20 pm

yeah you can't see primers on a gel. as for why your PCR didn't work, there can be many reasons. Could you give more details about your PCR protocol?
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seahow
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Post by seahow » Sun Apr 11, 2010 8:52 am

i think you should include a positive control.
that you say there are no primer is possible without amplification.

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