SDS-PAGE logMW plots anti-log?
Moderators: honeev, Leonid, amiradm, BioTeam
SDS-PAGE logMW plots anti-log?
i have been struggling to work out how i need to finish these calculations? how do i get the anti-log?
the standards from the SDS-PAGE have given me some molecular weights to do a graph, i have been told to plot logMW against distance run.
so i have calculated what the log MW's are eg/ 260kDa = 2.42 (logMW) and i have constructed the graph. giving me a slightly sigmoid shape.
so for example 2.42 was read off the graph for one of hte unknown bands how do i work out what the Mr is then is that just the anti-log and if so how do i do it?


the standards from the SDS-PAGE have given me some molecular weights to do a graph, i have been told to plot logMW against distance run.
so i have calculated what the log MW's are eg/ 260kDa = 2.42 (logMW) and i have constructed the graph. giving me a slightly sigmoid shape.
so for example 2.42 was read off the graph for one of hte unknown bands how do i work out what the Mr is then is that just the anti-log and if so how do i do it?



Re: SDS-PAGE logMW plots anti-log?

but now i have what the anti-log is how do i go from molecular weight to relative molecular mass, because the protien data sheet only has this info and they are all between 14,300 - 80,000 range




Who is online
Users browsing this forum: No registered users and 7 guests