BL21 (DE3) aggregates

About microscopic forms of life, including Bacteria, Archea, protozoans, algae and fungi. Topics relating to viruses, viroids and prions also belong here.

Moderators: Leonid, amiradm, BioTeam

Post Reply
sinadas
Garter
Garter
Posts: 2
Joined: Tue Oct 06, 2009 4:14 pm

BL21 (DE3) aggregates

Post by sinadas » Fri Mar 05, 2010 11:28 am

Hello everybody!

In our lab, we've prepared competent BL21 (DE3) cells with a standard protocol. During incubation at 37 ºC, after plasmid addition, we saw a pellet-like aggregate in the bottom of the eppendorf tube. Finally, the transformation didn't work, there were not any colonies in the plates. We don't know why it happens... Please, help us quickly because we need competent cells.

Thanks, ciao!

User avatar
JackBean
Inland Taipan
Inland Taipan
Posts: 5694
Joined: Mon Sep 14, 2009 7:12 pm

Post by JackBean » Fri Mar 05, 2010 4:00 pm

did you shake it?
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

Post Reply

Who is online

Users browsing this forum: No registered users and 1 guest