Bands on electrophoresis gel

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curiousgeorge12345
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Bands on electrophoresis gel

Post by curiousgeorge12345 » Wed Feb 24, 2010 9:53 pm

My question is very simple and yet I can not come up with an answer

When you digest DNA with 2 restriction enzymes, I would assume you would find three bands on the agarose gel. However, we're seeing only one band. Is that possible?

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JackBean
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Post by JackBean » Thu Feb 25, 2010 7:22 am

sure, if you get any number or different pieces of DNA of the same size (+- few nt), they will look like single band ;)
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

dinofernando
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Post by dinofernando » Sat Feb 27, 2010 10:36 am

Here's a question. If you have 100% digestion of vector, meaning your vector is linearized why can't RNApol synthesize protein when transformed into an e.coli cell?
-Dinesh
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JackBean
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Post by JackBean » Sat Feb 27, 2010 2:31 pm

maybe because RNA Pol does not synthesize protein ;)

You should not linearize the vector for bacteria transformation
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

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