Triton X-100 extraction

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kk
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Triton X-100 extraction

Post by kk » Wed Feb 24, 2010 2:31 pm

Someone please, what does it imply if a cytoskeletal protein is removed/retained after a mild Triton X-100 wash?

Seems to be a common technique yet no simple interpretaion of the results obtained from using this technique...

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JackBean
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Post by JackBean » Wed Feb 24, 2010 3:02 pm

Triton is detergent, it's supposed to cover the hydrophobic pathes of transmembrane proteins or proteins stacked together by them. It should also break the membranes efficiently.
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

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