Culturing Hela Cells

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Jasmine0507
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Culturing Hela Cells

Post by Jasmine0507 » Mon Feb 22, 2010 1:52 pm

Hi Guys,

I am very new to culture of mammalian cells and I have to design a protocol as a part of my Lab work.Can any one explain me how do we start a culture of HeLa cells from frozen cells.Which would be the best culture medium for Hela Cells ? Are these cells adherent or in suspension while in culture ?

Secondly , I will be transfecting these cells using three different techniques in 24-well plate. So, I would like to know
about transferring these cells to the 24-well and how to maintain them during transfection, how often to change the medium. Do we bring the cells in suspension before transfection ?

I have read several protocols but I have not very well understood. I hope you can make out from my questions that I have no experience of culturing mammalian cells.

Kindly help me.
Thank you.

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mith
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Post by mith » Tue Feb 23, 2010 4:16 am

you should google hela cell culture protocol
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