cell culture on PDMS

Discussion of all aspects of cellular structure, physiology and communication.

Moderators: honeev, Leonid, amiradm, BioTeam

Post Reply
Sohye
Garter
Garter
Posts: 4
Joined: Sat Feb 20, 2010 6:01 pm

cell culture on PDMS

Post by Sohye » Sat Feb 20, 2010 6:08 pm

How can I detach cells from surface of PDMS?
I did trypsin with EDTA treatment for two minutes but, it didn't work well.
Help me~ :D

User avatar
mith
Inland Taipan
Inland Taipan
Posts: 5345
Joined: Thu Jan 20, 2005 8:14 pm
Location: Nashville, TN
Contact:

Post by mith » Sun Feb 21, 2010 4:58 am

what's your cell type?
Living one day at a time;
Enjoying one moment at a time;
Accepting hardships as the pathway to peace;
~Niebuhr

Sohye
Garter
Garter
Posts: 4
Joined: Sat Feb 20, 2010 6:01 pm

Re:

Post by Sohye » Sun Feb 21, 2010 5:42 am

mith wrote:what's your cell type?

The cell type is HeLa.
Should I increase the time of trypsin with EDTA treatment more than 2min ?

User avatar
MrMistery
Inland Taipan
Inland Taipan
Posts: 6832
Joined: Thu Mar 03, 2005 10:18 pm
Location: Romania(small and unimportant country)
Contact:

Post by MrMistery » Sun Feb 21, 2010 4:57 pm

can't hurt to try. Also try gently tapping the flask after the digest, that used to work well when I did tissue culture (never used HeLa cells though)
"As a biologist, I firmly believe that when you're dead, you're dead. Except for what you live behind in history. That's the only afterlife" - J. Craig Venter

Sohye
Garter
Garter
Posts: 4
Joined: Sat Feb 20, 2010 6:01 pm

Post by Sohye » Mon Feb 22, 2010 2:39 am

But..It's not a flask. It's a kind of chip made of PDMS. So does the tapping work well in case of chip? It's too small...

User avatar
mith
Inland Taipan
Inland Taipan
Posts: 5345
Joined: Thu Jan 20, 2005 8:14 pm
Location: Nashville, TN
Contact:

Post by mith » Tue Feb 23, 2010 4:13 am

I've tried trypsin up to 20 mins with both mcf7 and rex cartilage cells without much problems, try incubating for longer(@37 C). If too many die you'll have to find another way.
Living one day at a time;
Enjoying one moment at a time;
Accepting hardships as the pathway to peace;
~Niebuhr

Post Reply

Who is online

Users browsing this forum: No registered users and 4 guests