when you isolate ribosomes, do you get the both subunits?

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sixpence
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when you isolate ribosomes, do you get the both subunits?

Post by sixpence » Fri Jan 29, 2010 5:37 pm

For example, if you isolate the ribosomes through centrifugation to get the sedimentation coefficient, would you get the large and small subunit separately, or together, or a mixture of both?

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JackBean
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Post by JackBean » Fri Jan 29, 2010 6:35 pm

IMHO depends on conditions. If you use conditions, which separate them, you get them separate and vice versa ;)
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

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JackBean
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Post by JackBean » Fri Jan 29, 2010 7:18 pm

I mean conditions, when they are not bonded together.

But are they bonded in the cell at all? Except on the mRNA of course.
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

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