amount of iron in spinach

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jwalin
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amount of iron in spinach

Post by jwalin » Thu Jan 21, 2010 11:52 am

hi
i don't know if this is in the right forum :( but anyways
i have to do an experiment about the amount of iron (II) ions in spinach.
i don't know how will i do this. there are 2 ways- chromatography and titration

1. using colorimetry -i make a standard curve using an iron tablet and undergo colorimetry the green color of the spinach pigment interferes with readings
2. using titration- the green pigment color interferes once again.

now i can do a few things.
1. if we can remove the iron ions and turn it to colorless (i can do this again in two ways-
a) using some reagent
or
b) make it a complex colorless)
again i don't know how to do any of these
then i can substract the colorimetric intensities. i don't know how to do this
2. i make the pigment colorless. a bad solution


any help is appreciated.
thanks in advance
it isn't what you do that matters but it is how you do it

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JackBean
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Post by JackBean » Thu Jan 21, 2010 2:23 pm

If will you make the iron colorless, how can you use the colorimetry?

How will you isolate it? It should get rid of the pigments by some organic solvents.

(BTW spinach is not very the best source of iron;)
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

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jwalin
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Post by jwalin » Thu Jan 21, 2010 3:54 pm

see i have 1 reading of pigment + iron and if i get a reading of pigment alone then the colorimetric reading of iron is the difference
i don't think i can remove the pigment if any is there.
it isn't what you do that matters but it is how you do it

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JackBean
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Post by JackBean » Thu Jan 21, 2010 4:04 pm

why not? extract it into some organics.
You could use EDTA.
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

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