Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.
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I have prepared SOB for competent cells preparation. According to the procedure, I have to add Mg solution (MgSo4.7H2O and MgCl2.6H2O) after autoclaving it (just before use). but by mistake, I added Mg solution before autoclaving it. I can always discard it and make a fresh SOB solution, but is there any other way through I could save the reagent from getting wasted. What would happen to divalent ions if I add them and then autoclave the solution?
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