Competent cell preparation

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jshreyas
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Competent cell preparation

Post by jshreyas » Thu Jan 14, 2010 6:36 pm

I have prepared SOB for competent cells preparation. According to the procedure, I have to add Mg solution (MgSo4.7H2O and MgCl2.6H2O) after autoclaving it (just before use). but by mistake, I added Mg solution before autoclaving it. I can always discard it and make a fresh SOB solution, but is there any other way through I could save the reagent from getting wasted. What would happen to divalent ions if I add them and then autoclave the solution?

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JackBean
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Post by JackBean » Thu Jan 14, 2010 9:47 pm

They will lose the water :lol: (that said one postdoc in our lab)

IMHO nothing.

BTW what's the difference between SOB and SOC?
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

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