longest spleen cells culture time

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jasmina
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longest spleen cells culture time

Post by jasmina » Tue Dec 15, 2009 7:47 am

hi there,
these days, i am worried about my spleen cells, i used same protocol as before, but after 7 days' culture, there are so few cells sticking to the bottom of petri dishes, i do not know why. usually, D1, i extract spleen cells and cultured in cell culture dishes, D2, collecte supnatant and seed in petri dishes, D4, feed spleen cells, and at d7, I can use spleen cells ( cells which sticking to the bottom of petri dishes), but this time, there are few cells sticking to the bottom of petri dishes.i want to culture these cells for a longer time, but i am worry about that, i don't know the longest culture time for spleen cells in vitro, is there anyone who can help me?
thanks a lot!

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