Help with my Beetroot Lab

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thezooguy
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Help with my Beetroot Lab

Post by thezooguy » Sun Dec 13, 2009 11:42 am

Hello, I need some help with this lab. We tested 10 slices of beetroot in 8 different substances.

1) 10 ml of distilled water (non-rinsed slices)

2) 10 ml of distilled water (rinsed slices)

3) 10 ml of HCL

4) 10 ml of NaOH

5) 10ml of Ethanol

6) 10ml of 40C water

7) 10 ml of 60C water

8) 10 ml of 80C water


I have a good understanding of why more pigment is released in the way it is for the temperatures, but I'm not sure how the pigment is released/changes color due to the HCL, NaOH, and ethanol.

Also, my teacher would like me to somehow graph the data, but I don't see how I can graph this since all I've gathered from the experiment is qualitative data.

All help is appreciated, thanks in advance =)

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JackBean
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Post by JackBean » Sun Dec 13, 2009 11:50 am

http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

thezooguy
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Re:

Post by thezooguy » Sun Dec 13, 2009 4:56 pm



Thanks for the links, but they don't have anything that I didn't know on here. I'm still confused on what happens with the NaOH, ethanol and HCL on the cellular level. I've somewhat got an idea of what happens with NaOH, but I'm not that certain of ethanol and especially HCL (since it turns into a bile-like color).

Darwin420
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Post by Darwin420 » Mon Dec 14, 2009 5:36 am

What were your qualitative analysis of the roots in HCL vs NaOH?

Ethanol will dissolve chlorophyll and obviously other parts of the plant. Ethanol is a good solvent.

HCl and NaOH are a strong acid and a strong base....define an acid and a base and then think about it.

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JackBean
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Post by JackBean » Sun Dec 20, 2009 1:17 pm

The point of my links was, that these colors and their colors are pH-dependent, so if you use acid or hydroxide, the color of extract will be different.

I think, Darwin420 has a good point about EtOH and chlorophyll.

BTW for both, it is supposed to be HCl!!! HCL would be some substance composed of hydrogen, carbon and some element abbreviated L :roll:
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

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