is this correct????

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Bill123
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is this correct????

Post by Bill123 » Mon Nov 23, 2009 4:31 am

I am writing the thesis, anybody can tell me are there some mistake in the following sentence?? Thank you!!

The pUC19-p450 and pUC19-p450-bm3 plasmids containing p450 and p450-bm3 genes respectively were used as templates for PCR amplification using primers pYES-For (5’- ATGCATGCATGCATGCATGCATGC-3’) and pYES-Rev (5’- ATGCATGCATGCATGCATGCATGC-3’), both of which anneal to the pUC19 vector.

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JackBean
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Post by JackBean » Mon Nov 23, 2009 9:35 am

Except, that both your primers seem tzo have the same sequence, it looks fine to me.
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

Bill123
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Post by Bill123 » Mon Nov 23, 2009 9:11 pm

JackBean wrote:Except, that both your primers seem tzo have the same sequence, it looks fine to me.



:D Thank you for your help!!

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MrMistery
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Post by MrMistery » Thu Nov 26, 2009 6:22 pm

the fact that both your primers are identical means you got something wrong
"As a biologist, I firmly believe that when you're dead, you're dead. Except for what you live behind in history. That's the only afterlife" - J. Craig Venter

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