role of donor vector in gene cloning

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roniadam
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role of donor vector in gene cloning

Post by roniadam » Sun Nov 22, 2009 10:30 am

Hi

I would like to know why do we use a donor vector when we clone a desired gene to introduce it into a destination vector for expression analysis? why we don't go directly to introduce our gene into the destination vector without recombining it into the donor vector?

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JackBean
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Post by JackBean » Sun Nov 22, 2009 12:42 pm

I guess, you are talking about some system like GATEWAy, right?
Well, in this system, you use only one cloning, that into the entry vector and from these you can recombine it into many other vectors, you can do overexpression, fusion at N- or C-end with any peptide etc. etc. :wink:
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

roniadam
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Post by roniadam » Wed Nov 25, 2009 3:10 am

Thanx Jack

you r right. I am talking about the gateway system. Thank u for your very useful info.

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