Fc fusion probes

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sophie1983
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Fc fusion probes

Post by sophie1983 » Thu Nov 19, 2009 9:42 am

Hi I was wondering if anyone could tell me how Fc/AP fusion probes work? Can you use them as you would in a normal in situ hybridisation to see expression patterns? Also what are the benefits to using them?

I've tried searching the net for this but there seems to be a real lack of basic information.

Any explains would be great!

Thanks :D

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JackBean
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Post by JackBean » Thu Nov 19, 2009 10:05 am

I guess Fc is the part of antibody molecule, right? AP is alkaline phosphatase?
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

sophie1983
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Post by sophie1983 » Thu Nov 19, 2009 2:44 pm

Yep

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JackBean
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Re: Fc fusion probes

Post by JackBean » Thu Nov 19, 2009 3:00 pm

Are you sure, this is supposed to work for hybridization? I could imagine this for purification, but it has no active specific binding site for hybridization... :?
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

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