biochemistry-enzyme kinetics lab help

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Garter
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biochemistry-enzyme kinetics lab help

Post by nickname » Thu Nov 19, 2009 3:16 am

Hello,

I had to do a lab on enzyme kinetics: finding Vmax and Km for alkaline phosphatase.

What are some sources of error that could be found when conducting this kind of experiment?

I know that temperature could be one of the errors, but could you please help me find other types of errors encountered in enzyme kinetics experiments?

Thanks for your help!

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JackBean
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Post by JackBean » Thu Nov 19, 2009 4:10 am

E.g. pH. Like if you set pH of your buffer to the optimal pH, but it's not strength enough and some of the components added to reaction will change it. Also, the reaction itself can change pH (that's why you use buffers;) and especially phosphatase reaction will change it) and if it changes pH in the direction, where is your buffer weakened, than you are in trouble ;)
Of course, bad pipeting, e.g. if you dilute your substrate wrong, you will get bad results ;)
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Post by nickname » Wed Nov 10, 2010 10:51 pm

Thank you so much for your help!

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