Benedicts solution in a acidic medium (by accident!)

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mahela007
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Benedicts solution in a acidic medium (by accident!)

Post by mahela007 » Wed Nov 18, 2009 3:50 pm

Hi.. This is my first post on these forums.
I wad doing the test for reducing sugars using Benedicts reagent. To test for sucrose, the solution should be heated with HCl, and neutralized with a base before benedicts reagent is added.
I forgot to neutralize with the base. ;-)
How does benedicts reagent behave in an acidic medium? Will it still indicate the presence of reducingsugars?
The characteristic phrase which accompanies new discoveries is seldom "Eureka!". More often it's "Hey, that's odd!"
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JackBean
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Post by JackBean » Thu Nov 19, 2009 2:10 am

Well, I found only, that it's composed from sodium citrate and carbonate and copper sulphate, nothing about HCl, which would relase CO2 from the solution, so I don't know...

My guess is that the basic enviroment is needed in order to precipitate the Cu(I) oxide, which could stay dissolved in acidic solution as some salt.
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jwalin
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Post by jwalin » Thu Nov 19, 2009 12:48 pm

the last part of the jackbean's post is what i think

but the base is used only to nuetralise the acid not make the solution basic
it isn't what you do that matters but it is how you do it
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