Real-time PCR&Reverse transcription PCR

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Elva
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Real-time PCR&Reverse transcription PCR

Post by Elva » Thu Nov 05, 2009 2:41 am

Such as subject ,when we see qRT-PCR,what is the really meaning of "RT"?
What are the difference between them? :)

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mith
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Post by mith » Thu Nov 05, 2009 4:40 am

look it up
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JackBean
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Post by JackBean » Thu Nov 05, 2009 4:47 am

RT can be both reverse transcriptase as well as real-time ;)
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

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qqsvery
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Post by qqsvery » Thu Nov 05, 2009 12:59 pm

Real-time PCR, also known as kinetic PCR, qPCR, qRT-PCR and RT-qPCR, is quantitative PCR method for the determination of cope number of PCR templates such as DNA or cDNA in a PCR reaction. There are two flavors of real-time PCR: probe-based and intercalator-based.

I`m sure you will be interesting to look into this website to learn more about it!Here it is:
http://www.protocol-online.org/prot/Mol ... index.html
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canalon
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Post by canalon » Thu Nov 05, 2009 2:25 pm

To sum everything up, due to the 2 different possible meaning of RT-PCR, people are gradually shifting to q for quantitative, instead of Real-Time. So it becomes clearer to describe what is done:
qPCR: quantification of DNA
RT-PCR: amplification of RNA
qRT-PCR: quantification of RNA
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