Question about restriction enzyme mapping

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jocelyn074
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Question about restriction enzyme mapping

Post by jocelyn074 » Thu Oct 29, 2009 1:22 am

Hi,

I am working on this problem as practice for my exam next week and i am stuck on this one. The question is:Someone gives you a plasmid, you digest it with Pst1 and get 3 bands that are 6kb, 4kb, and 3kb. You digest with Xho and get 1 band that is 4 kb long.

Ok so I was thinking that it would be 13kb long with the numbers from the Pst1 enzymes. But I was thinking that if Xho had only 1 cut that the band would be more like 13 kb long. I don't really understand the numbers so I must be doing it wrong.

I thought the addition of the bands=the plasmid size so I don't know why the band from xho is only 4kb.

Can somone please help?

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JackBean
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Post by JackBean » Thu Oct 29, 2009 2:10 am

What about, if Xho digest three-times? Always like 4 kbp away from each other? ;) So you will get one band with three pieces actually
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

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