Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.
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- Posts: 24
- Joined: Tue Sep 18, 2007 8:41 am
I want to check the cleavage of a protein. The fragments I expect are 54 kDa and 8 kDa...
8 kDa is very small
Is it possible to see this small thing on a sds-gel???
- Inland Taipan
- Posts: 5694
- Joined: Mon Sep 14, 2009 7:12 pm
Sure, why not? You just have to choose the right concentration of AA/BIS (maybe use gradient, to have good resolution at both 8 and 54 kDa;), use silver staining and maybe add a little bit more of protein to see it
Cis or trans? That's what matters.
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