protein gel

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piefke
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protein gel

Post by piefke » Wed Oct 28, 2009 12:13 pm

Hi everybody!

I want to check the cleavage of a protein. The fragments I expect are 54 kDa and 8 kDa...
8 kDa is very small :( Is it possible to see this small thing on a sds-gel??? :shock:

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JackBean
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Post by JackBean » Wed Oct 28, 2009 1:10 pm

Sure, why not? You just have to choose the right concentration of AA/BIS (maybe use gradient, to have good resolution at both 8 and 54 kDa;), use silver staining and maybe add a little bit more of protein to see it ;)
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

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