Freezing Het-1A cells

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lrarias
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Freezing Het-1A cells

Post by lrarias » Mon Oct 26, 2009 5:40 pm

I recently obtain via ATCC Het-1A cells. Their product info sheet says that I must use the following cryoprotectant medium: L-15 plus 10% FBS, 1% PVP, and 7.5% DMSO. I did order the specific complete growth medium BEGM kit but I want to know if I can just DMSO to this growth medium in order to freeze or if it is necessary to get the cryoprotectant medium?

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MrMistery
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Post by MrMistery » Fri Oct 30, 2009 2:31 pm

in my tissue culture days i used to just add DMSO to whatever media I normally grew the cell lines, and it worked for the lines I worked with. However, i never worked with Het-1A, it may be that this particular line is more sensitive
"As a biologist, I firmly believe that when you're dead, you're dead. Except for what you live behind in history. That's the only afterlife" - J. Craig Venter

givaz
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Re: Freezing Het-1A cells

Post by givaz » Thu Nov 25, 2010 9:35 pm

Hi! I don't know if this is going to be any help at this stage but I work with this cell line and I freeze them in 90%FBS and 10% DMSO. This works really well for me as long as you remove, by centrifugation, the above mentioned mixture when you defrost the.
I have now been using this method for 3 years, I did try to use a commercially available freezing solution but it actually reduced my cell viability when reviving cultures.
Hope this helps someone out there : )

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Post by Natalya2 » Fri Nov 26, 2010 9:19 pm

10% DMSO in 90% FBS or growth media should be fine. are you using Mr Frosty or control rate freezer?

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