OD and bacterial cell number

About microscopic forms of life, including Bacteria, Archea, protozoans, algae and fungi. Topics relating to viruses, viroids and prions also belong here.

Moderators: honeev, Leonid, amiradm, BioTeam

Post Reply
chidu
Garter
Garter
Posts: 2
Joined: Wed Oct 21, 2009 7:44 pm

OD and bacterial cell number

Post by chidu » Wed Oct 21, 2009 8:06 pm

what is the relationship between the OD of the cell suspension and cell number, if i need 10 to the power 6 number of cell per ml than what should be the OD for that, is their any formula for that

User avatar
canalon
Inland Taipan
Inland Taipan
Posts: 3909
Joined: Thu Feb 03, 2005 2:46 pm
Location: Canada

Post by canalon » Wed Oct 21, 2009 9:06 pm

Yes , usually, but it varies with the organism. Usually you will run a culture and calibrate that way.
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)

chidu
Garter
Garter
Posts: 2
Joined: Wed Oct 21, 2009 7:44 pm

Re: OD and bacterial cell number

Post by chidu » Thu Oct 22, 2009 5:21 pm

yes it depends on type of cell for example E.coli than how to go for it , i need a formula for that

User avatar
canalon
Inland Taipan
Inland Taipan
Posts: 3909
Joined: Thu Feb 03, 2005 2:46 pm
Location: Canada

Post by canalon » Fri Oct 23, 2009 3:42 pm

Grow your bug
Measure OD
plate appropriate dilution in triplicates and incubate
Count
Divide OD by cell number: here you are.
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)

lizier
Garter
Garter
Posts: 5
Joined: Tue Nov 03, 2009 6:27 am

Post by lizier » Thu Nov 05, 2009 3:33 am

I think you can get the formula by counting.
you should select different concentration culture by dilution, measure the OD each of them,then plate each of them ,and count them.in this ,you can get a equation.

lizier
Garter
Garter
Posts: 5
Joined: Tue Nov 03, 2009 6:27 am

Post by lizier » Thu Nov 05, 2009 3:38 am

my English is very poor, maby my words are fillde with mistake,I'm sorry about that,Wish you can understand me.

Post Reply

Who is online

Users browsing this forum: No registered users and 1 guest