BAC linearization

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Nodo
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BAC linearization

Post by Nodo » Mon Oct 12, 2009 3:16 pm

Hi,

I just finished my BAC modification to create a new transgenic mouse line. Now I have to purify this construct to inject it to zygotes. I know that it's better to linearize the BAC before injection however, I wondered if it is a problem if the injected construct still contain the antibiotic resistance gene which is in the Backbone.

Thanks for your Help!

Nodo

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