bacteriophages

About microscopic forms of life, including Bacteria, Archea, protozoans, algae and fungi. Topics relating to viruses, viroids and prions also belong here.

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taata
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bacteriophages

Post by taata » Fri Jul 08, 2005 9:12 pm

Draw fully labelled diagram, and write some explanatory notes, explaining how you would quantify the number of bacteriophages in an unknown suspension. :cry:

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mothorc
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Post by mothorc » Mon Jul 11, 2005 12:11 pm

you must have 2 things:
+a TEM microscopic to have a view
+a solution of micro marbles that you know the concentration(y)
Then disolve them with an equal volume and count on microscopic.(see a phage, b micromarble)
Finally solve the function x=ab/y .
I will find the name of this method for you.
:mrgreen:

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canalon
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Post by canalon » Mon Jul 11, 2005 3:14 pm

mothorc wrote:you must have 2 things:
+a TEM microscopic to have a view
+a solution of micro marbles that you know the concentration(y)
Then disolve them with an equal volume and count on microscopic.(see a phage, b micromarble)
Finally solve the function x=ab/y .
I will find the name of this method for you.
:mrgreen:


There are easier solutions, that do not need any microscope: search for plaque assay? counting.

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mothorc
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year

Post by mothorc » Tue Jul 12, 2005 2:09 am

terible sorry
I forget the easiest
but what will you do if your virus don't have plaque (special animal or plant virus)

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canalon
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Re: year

Post by canalon » Tue Jul 12, 2005 1:48 pm

mothorc wrote:terible sorry
I forget the easiest
but what will you do if your virus don't have plaque (special animal or plant virus)


First, related to the question at the beginning of the thread, I do not think you have to take this into account: He's talking about bacteriophage, You just a sensitive strain of bacteria, the one used for propagating the phage would be good :)
Then if you cannot use plaque, your method seem good to me, although a real pain to prepare compared to the other...

Patrick

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mothorc
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Post by mothorc » Wed Jul 13, 2005 12:58 pm

ok ok I see

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