BSA conjugation

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vsun
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BSA conjugation

Post by vsun » Mon Sep 28, 2009 1:14 am

What happens during the process of conjugating BSA to oligos? In other words, what is the science behind it?

Okay, so I'm doing a research report. I followed a protocol for the modification of BSA. I want to see if conjugating BSA to oligos probes will increase the sensitivity of the results in my DNA microarray chip. So far, I conjugated BSA to oligos (powder that was dissolved in water) and also conjugated BSA to SANH. Since oligos is relatively small and BSA is a large protein, I hoped to increase the sensitivity. After running a protein gel, I saw that the well that contained BSA conjugated to the oligos shifted upwards compared to the well that had BSA conjugated to SANH.
Therefore, I can conclude that the oligos did get larger after conjugating with the BSA, and that they did bind. Hopefully, this will increase the sensitivity of my results when I conjugate BSA to the oligos probes on the DNA chip. (By the way, we are designing DNA chips for efficiently genotyping HPV strains.)

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