How to know the sequence of specific gene and producing it ?

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DLOFI
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How to know the sequence of specific gene and producing it ?

Post by DLOFI » Sun Sep 27, 2009 11:34 pm

we have a human gene of 500 bp which is responsible for production of a specific antibody for a certain infection

we need
1. to know its sequence
2. produce it commercially as a pure human antibody to use it as treatment for this infection .

what are the techniques i have to use to achieve this goals .
in detail plz as much as u can


many thanks for every 1thinking to help me.

d lofi

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MrMistery
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Post by MrMistery » Mon Sep 28, 2009 3:25 am

are you for real? If a gene is just 500 bp you can just sequence it directly (dideoxy method allows for an 800 bp fragment to be sequenced).
To produce it commercially is a lot tricker, hybridoma cells are probably the way to go. unfortunately because antibody molecules are so big and complex (and as a consequence so much larger than 500 bp) you can't just put them in E.coli and wait for the bugs to make it, like you can do with insulin
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mith
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Post by mith » Mon Sep 28, 2009 3:36 am

Ok, here's what you do:
1.you sequence the human DNA which has already been done.
2. Build a supercomputer capable of performing LOTS of calculations.
3. Design an optimized program for predicting gene products based on the DNA sequence.
4. Design another program to fold the proteins from #3 into the correct shape. Don't forget VDJ rearrangements!
5. Use a dock program to fit a model of the antibody to the proteins from #4.
6. Take the best match from #5 and that's your sequence.

To produce the antibody, just use supercomputer from #2 to build a replicator.
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JackBean
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Post by JackBean » Mon Sep 28, 2009 11:32 am

mith: that's some kind of joke?
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

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mith
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Post by mith » Wed Sep 30, 2009 2:01 am

Are you saying it wouldn't work?
Living one day at a time;
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Accepting hardships as the pathway to peace;
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