Amino Acid Titration

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micron66
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Amino Acid Titration

Post by micron66 » Tue Sep 22, 2009 7:01 am

Hello.

I´m having problems understanding some of the key concepts of amino acid titration - I hop you can help me.

As I understand it, the amino acid is at it´s fully protonated form when the solution is at it´s lowest pH value. How can this be, when the definition of a low pH value is a high concentration of H+? Why isn´t the amino acid fully deprotonated instead? And when the amino acid is at it´s highest pH value - why is it fully deprotonated instead of fully protonated? Now that the amino acid is fully deprotonated, the solution must be full of H+-ions, and therefore the pH should be very low.

And one more thing: The amino acids that are negatively or positively charged at neutral pH - what happens to them during titration?

Hope you can help me. Thank you.

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JackBean
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Post by JackBean » Tue Sep 22, 2009 7:14 am

That's all about equilibria.
Lets have some simple system with simple acid

HA <-> H+ + A-

if you add protons to the mixture, what will happen? You will have lot of protons, so the equilibrium will move to the left. And that is, why is amino acid protonated at low pH. The acidifying protons do not come from the amino acid (otherwise it would be at approx. neutral pH).
The same applies at high pH, just reversed.

About the charged AAs, they behave in accordance to they pK. If you have pH under it, the group will be protonated, if will be higher, the group will be deprotonated. For the same reasons as above ;)
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

micron66
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Post by micron66 » Tue Sep 22, 2009 6:03 pm

Thank you. I got it.

elyateem
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Re: Amino Acid Titration

Post by elyateem » Sat Apr 24, 2010 8:09 am

hi
please...could anyone help me???
i have a problem in calculating the net charge for the naa,aaa,baa
i dont know where we put +.5,-.5,+1or -1
plzzzzz help me

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JackBean
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Post by JackBean » Sat Apr 24, 2010 8:40 am

what is naa,aaa,baa? :roll:

where does the stupid practice, not to write 0, comes from? :roll:
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

elyateem
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Re: Amino Acid Titration

Post by elyateem » Sat Apr 24, 2010 8:49 am

naa=neutral amino acid
aaa=acidic amino acid
baa=basic amino acid
......its not stupid

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JackBean
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Post by JackBean » Sat Apr 24, 2010 8:58 am

it is pretty stupid to write +.5, that's nothing

just look, what charged groups it have at the particular pH
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

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Post by elyateem » Sat Apr 24, 2010 9:21 am

iam not talking of ma own pocket
its written like this in the book
if you dont know wt iam talkin about so dont answer without say thats this is stupid question

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JackBean
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Post by JackBean » Sat Apr 24, 2010 9:48 am

I'm saying, it's stupid to write like +.5, not that the question is stupid (not that it wouldn't be...)

I gave you the answer...
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

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