Protein half-life

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tomerheb
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Protein half-life

Post by tomerheb » Wed Sep 16, 2009 1:48 pm

Hello,
I'm trying to see the rate of degradation of a certain protein in 293 cells and i want to compare my results to known protein's half-life, for example actin or another abundant cellular protein.
Does anyone have information regarding the half-lives of a very slow degrading proteins?
Thank you,
Tom.

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JackBean
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Post by JackBean » Wed Sep 16, 2009 6:36 pm

You mean very slow degraded proteins? :-P

What information you mean?
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

MJD
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Re: Protein half-life

Post by MJD » Wed Sep 30, 2009 11:21 pm

What's the protein?

The only problem I see is that you will be assaying the protein steady-state (synthesis-degradation). How do you plan to control for this? Most likely, if it is a well known protein, then you can do a literature search to find the half-life and a method for determining the half-life of the protein in your particular system relative to what someone else has already done.

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MrMistery
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Post by MrMistery » Thu Oct 01, 2009 2:30 am

Histone H2B has a reported half-life of 223 days. Also, a nuclear pore component called Nup107 is not degraded at all in postmitotic cells. Slowly degraded enough for you?
"As a biologist, I firmly believe that when you're dead, you're dead. Except for what you live behind in history. That's the only afterlife" - J. Craig Venter

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