Western Blot from an Agarose gel...?

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herm913
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Western Blot from an Agarose gel...?

Post by herm913 » Thu Aug 13, 2009 12:56 pm

Hello all,
i made a 4% agarose gel for protein separation using 1x tgs buffer. I was wondering if it would be pointless (or even detrimental) to perform a Western blot on the same gel, just to visualize it better.
help? Thanks

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MrMistery
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Post by MrMistery » Fri Aug 14, 2009 6:20 pm

I've never heard of anyone running proteins through an agarose gel before (why are you doing it btw?). There isn't any mechanistic aspect to blotting the gel (except maybe that it is too thick to fit into a western transfer machine), but I think you would have a very hard time figuring out the right parameters (current, voltage, time) at which to transfer it.
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mith
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Post by mith » Mon Aug 17, 2009 10:40 pm

ummm, the holes are too wide?
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Accepting hardships as the pathway to peace;
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MrMistery
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Post by MrMistery » Thu Aug 20, 2009 9:26 pm

what do you mean?
"As a biologist, I firmly believe that when you're dead, you're dead. Except for what you live behind in history. That's the only afterlife" - J. Craig Venter

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