maping and linkage problems

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hemu0912
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maping and linkage problems

Post by hemu0912 » Sun Jul 26, 2009 5:59 pm

if the E. coli genome is 4 X 106 bp, answer the following questions.?
1.If the E. coli genome is 4 X 106 bp, answer the following questions.
a. If this circular molecule was made linear, how long would it be in
cm?
b. In a single E. coli cell, what is the weight (in μgrams) of this DNA?
c. If you extracted chromosomal DNA from a culture of E. coli and had
200 μgrams, how many moles would you have of this molecule?

2.Knowing the genome size of E. coli and the fact that it can divide ca.
every 20 minutes (under optimal growth conditions), calculate the
rate of DNA replication in bp/sec (assume only one origin of
replication and bidirectional replication).

3. Explain how and why the following circumstances influence
characteristics of temperature-induced DNA melting.
a. Percentage of GC base pairs
b. Urea (forms hydrogen bonds with bases)
c. Sodium chloride (neutralizes negatively charged phosphates)

Friends please help me out. i dont get any of those answers. :(
Thanks :D

hemu0912
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Post by hemu0912 » Sun Jul 26, 2009 9:45 pm

NO REPLY YET.......:( :(

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canalon
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Post by canalon » Mon Jul 27, 2009 11:09 am

Read the rules
Do your own homework.
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)

reyhane
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Post by reyhane » Mon Aug 03, 2009 12:22 pm

1.

10 bp = 3.4 nanometers

4 X 106 bp / 0.34 nanometers = 1.36 x 106 nanometers = 1.36 x 10-1 cm !!

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