Methods Identifying Unknown Bacteria?

About microscopic forms of life, including Bacteria, Archea, protozoans, algae and fungi. Topics relating to viruses, viroids and prions also belong here.

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damm182
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Methods Identifying Unknown Bacteria?

Post by damm182 » Mon Jul 20, 2009 5:07 am

Hi can anyone give me a list of experiements that I can do to find out an unknown bacteria sample? thanks :D

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canalon
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Post by canalon » Mon Jul 20, 2009 10:51 am

16S DNA sequencing...
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Post by MrMistery » Wed Jul 22, 2009 2:37 am

short and efficient list there canalon :)
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Post by kolean » Wed Jul 22, 2009 12:42 pm

Isn't the first thing to do is a Gram stain? And then find out if it is anaerobic, aerobic, or facultative? then look at it under a microscope for its shape? what about the media that it can grow on? put different antibiotics in the media and see if it still can grow on it?

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Post by canalon » Wed Jul 22, 2009 4:27 pm

Of course kolean, you are right this is a good way. Although I would disagree with the use of antibiotics as they are not species specific. Although the microscopic observation (gram, shape, capsule...) is usually done after the bacteria has been grown so you should know by then if it is aerobic or not. Then a group of selective media either prepared by hand or in a kit (Biomerieux API, BD crystal...) selected on the basis of the first observations.

But my method works well too. I f you have the right equipment. Might be faster too.
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Re: Methods Identifying Unknown Bacteria?

Post by stochastic » Fri Jul 24, 2009 2:00 am

Gram's stain is certainly useful in classification but not always truthful. Some organisms, Acinetobacter in particular, can readily stain gram-positive in fresh clinical material, yet stains gram-negative after growth on plated media.
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Re: Methods Identifying Unknown Bacteria?

Post by zami'87. » Wed Sep 16, 2009 1:27 am

There's also API method for biochemical characteristics http://en.wikipedia.org/wiki/Analytical_Profile_Index
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Post by JackBean » Wed Sep 16, 2009 6:16 am

kolean wrote:Isn't the first thing to do is a Gram stain? And then find out if it is anaerobic, aerobic, or facultative? then look at it under a microscope for its shape? what about the media that it can grow on? put different antibiotics in the media and see if it still can grow on it?


Might be, but this takes lot of time, you need to be skillfull and often you get only the genus.
Basically, many PCR methods might be usefull and as wrote canalon, 16S sequencing is used, as it is specific and you do not need to cultivate the bacteria (so you do not risk the contaminaton or that the bacteria won't grow)
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Re: Methods Identifying Unknown Bacteria?

Post by kyra13 » Mon Aug 01, 2011 7:43 am

Go look in the back of your Microbiology text book at the flow chart. Or go to the library and get a copy of Bergey's Manual of microbiology

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