DNA hybridisation

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bizy
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DNA hybridisation

Post by bizy » Tue Jun 30, 2009 6:00 am

Can anyone help me here Iam struggling to understand how to calculate the amount of DNA probe i need for my hybridization solution in a blot.
say for a hybridization solution of 450 microliter, I have 0.5pmol DNA probe, what volume of the probe i need for this hybridization solution?

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