please help with dilutions?

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magentacatz
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please help with dilutions?

Post by magentacatz » Tue Jun 30, 2009 12:04 am

Please help? :?
I've been trying to understand this for hours but for some reason I just do not understand serial dilutions :(


I have a stock solution of 0.25 miligrams/mL and I have to get it to a concentration of 100 nanograms/mL and then also to 10 nanograms/mL.

I've done the whole C1V1 = C2V2 thing but it does not work for serial dilutions. I never learned about serial dilutions in college and now I have to do it in lab.

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MrMistery
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Post by MrMistery » Tue Jun 30, 2009 2:18 am

you set c1=0.25 miligrams/mL
c2=100 nanograms/mL
v2=whatever volume you need of your 100 nanograms/mL solution.

then you calculate your v1.

Once you have your 100 nanograms/mL you can do the following thing: use the same formula, setting c1=100 nanograms/mL, c2=10 nanograms/mL and v2=whatever volume you need of the 10 nanograms/mL solution, and then calculate v1 (in this particular case, you will only need to dilute your 100 nanograms/mL solution 10 times).

Cheers,
Andrei
"As a biologist, I firmly believe that when you're dead, you're dead. Except for what you live behind in history. That's the only afterlife" - J. Craig Venter

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