Lentivirus vector propagation

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roniadam
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Lentivirus vector propagation

Post by roniadam » Mon Jun 29, 2009 7:11 am

Hi

I bought a lentivirus vector containing alpha globin gene and I want to grow this vector. In what bug I should propagate it?

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MrMistery
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Post by MrMistery » Tue Jun 30, 2009 2:20 am

i'm an yeast person, so I am totally out of my element, but I would just look at what species that virus species normally infects and then start from there.
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canalon
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Post by canalon » Tue Jun 30, 2009 3:38 pm

Did the cell (line) in which the virus is effective not come with your virus? That should be basic customer support. I am sure that you are just a phone call (to whoever sold you the lentivirus) to know which cells to use. And by the way lentivirus AFAIK will be used in eukaryotic cell lines, not bacteria.
Patrick

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any proof. (Ashley Montague)

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MrMistery
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Post by MrMistery » Wed Jul 01, 2009 1:52 am

yeah, definitely, probably a cell line from some mammal... I think HIV is a member of the lentiviridae
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canalon
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Post by canalon » Wed Jul 01, 2009 2:12 am

Yes it is. It is also used as a vector in some case. But this is a large family, hence my answer.
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)

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