G genes

Debate and discussion of any biological questions not pertaining to a particular topic.

Moderators: honeev, Leonid, amiradm, BioTeam

Post Reply
Posts: 1
Joined: Sun Jun 28, 2009 7:16 pm

G genes

Post by zhetimxu » Sun Jun 28, 2009 7:21 pm

Hi, I am doing a science fair project, and there is a lot of background information that I need to understand before doing the project. I'm wondering if anyone knows any useful websites or if anyone can explain certain concepts to me. The objective of my project is to create a GFP-Protein G chimeric protein for immunological applications. I have already done some research on how PCR (polymerase chain reaction) works. However, I still do not understand the following things:

- Protein G genes
- Recombinant His-tagged proteins (and how they are purified by affinity chromatography on a nickel-agarose column)

Thank you very much for your help!

- Tim

Posts: 345
Joined: Thu Jun 25, 2009 3:15 am

Re: G genes

Post by kolean » Mon Jun 29, 2009 2:20 pm

Though Wikipedia does get a bad rap sometimes, I have found it useful to get some basic info on science related terms.

Thus for the G protein: http://en.wikipedia.org/wiki/G_protein# ... G_proteins

the recombinant His-tagged proteins is a under this: http://en.wikipedia.org/wiki/Polyhistidine-tag

Hope this helps you to get started.

Post Reply

Who is online

Users browsing this forum: No registered users and 17 guests