Anybody knows how stable is ssDNA??

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primary
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Anybody knows how stable is ssDNA??

Post by primary » Tue Jun 09, 2009 11:08 pm

Hi guys,
I'm preparing the probe for in situ hybridization. It's the product from degenerated PCR so i guess it's ssDNA. I labeled the probe with DIG, keep in 50%formamide and hybridization buffer with carrier DNA. I was so stupid that I left it overnight at my bench. Anybody has and information about how stable is ssDN at room temperature? especially in that buffer? Please help, my background in biochemistry is not that strong. Thanks a lot.

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MrMistery
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Post by MrMistery » Fri Jun 12, 2009 7:20 pm

i would say you may have degradation. A quick thing you can do is run a small quantity of it on a gel, and see if it is all the same size. If you get a smear, you had degradation.
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Re: Anybody knows how stable is ssDNA??

Post by lihang113 » Thu Jun 18, 2009 8:32 am

hydrogen bond

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