ATG8/LC3 western-blotting

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zky
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ATG8/LC3 western-blotting

Post by zky » Tue May 05, 2009 6:50 pm

Hi guys,

I am doing LC3 western-blotting. Some, but not all, papers used E-64d and Pepstatin A containing HBSS to starve cells before collecting and lysing them. I am wondering if E-64d and Pepstatin A are required for LC3 western-blotting. Could these drugs cause anything artificial?

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