Molar Extinction Coefficient Trouble!

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sophi3nutt
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Molar Extinction Coefficient Trouble!

Post by sophi3nutt » Tue Apr 21, 2009 11:38 am

I have a graph of absorbance against concentrations of BCG dye at 614 nm.
I have been asked to estimate the molar extinction coefficient for BCG from the graph and show how my value was calculated. The Molecular weight values for BCG is 698.

I simply do not know where to start!
So far:
E=A/c
I have an absorbance of 0.406/0.35 = 1.16 L.mol-1.cm-1...but do not know what to do regarding the molecular weight.

alexandertg6
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Post by alexandertg6 » Wed Apr 22, 2009 12:31 pm

nothing. ignore it. A=EbC where E is molar extinction coefficient, b is the path of light in cm, and C is concentration

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