B-per GST fusion protein purification

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bsengez
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B-per GST fusion protein purification

Post by bsengez » Mon Apr 13, 2009 8:36 pm

I need to purify GST tagged protein by using B-per reagent from pierce.(I used pGEX2T vector).Is there anyone used this kit? I have used Y-Per reagent before and I have loads of problems. My protein yield was so low. I had also low amount GST band except GST tagged protein on SDS. I don’t want to face with these problems again. I will use this protein to immunize rabbit.
Thanks in advance,
Burcu

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