bacterial growth curves and cell numbers

About microscopic forms of life, including Bacteria, Archea, protozoans, algae and fungi. Topics relating to viruses, viroids and prions also belong here.

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am2lab
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bacterial growth curves and cell numbers

Post by am2lab » Fri Apr 10, 2009 5:31 pm

Does anyone know if the numbers of cells at the same OD600s are different between different Escherichia coli strains (when they are grown under the same conditions), or are they same? I would think that the relationship between OD600 and numbers of cells would depend on cell size... Is that correct? Do I have to measure ODs and count cells for different strains or can I just do cell counts for one and measure ODs for all to know how many cells are there in the other strains? Please help!

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canalon
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Post by canalon » Sat Apr 11, 2009 3:55 am

They are different. Making a calibration of the OD is a good idea when working with different strains. However, unless working with very bizarre strains, the OD will give you a good idea of the cell number +/- 1 order of magnitude. It can be enough precision or not depending on what you want to do
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)

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