Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.
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- Posts: 11
- Joined: Tue Jan 22, 2008 11:57 am
After you "cut" DNA fragments using restriction enzymes, and then are "pasting" them together using DNA ligase...
do you need to select a specific DNA ligase that matches with the specific restriction enzyme that was used?
- Posts: 364
- Joined: Sat Dec 31, 2005 12:45 am
- Location: Manchester
No. It'll anneal any broken strand. The vector is prevented from self-ligation by alkaline phosphatase.
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