DNA ligases

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SysBio
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DNA ligases

Post by SysBio » Fri Mar 27, 2009 3:40 am

After you "cut" DNA fragments using restriction enzymes, and then are "pasting" them together using DNA ligase...

do you need to select a specific DNA ligase that matches with the specific restriction enzyme that was used?

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Sepals
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Post by Sepals » Fri Mar 27, 2009 11:44 am

No. It'll anneal any broken strand. The vector is prevented from self-ligation by alkaline phosphatase.
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