Percoll and RediGrad Look alikes

About microscopic forms of life, including Bacteria, Archea, protozoans, algae and fungi. Topics relating to viruses, viroids and prions also belong here.

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palpha3
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Percoll and RediGrad Look alikes

Post by palpha3 » Thu Jun 30, 2005 5:47 pm

Hi All,

I'm working on being able to seperate vegetative bacterial cells from their spore form. I've used Percoll and my results are great. I've read RediGrad is the newest version of Percoll. However, both are way too expensive for my application so I cant keep using these mediums.

My question is basically, does anyone know of another medium that will work as well as Percoll but cost a whole lot less? I need a density of about 1.2-1.3 to seperate what i'm working on. Any help would be greatly appreciated.
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palpha3
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Post by palpha3 » Tue Jul 05, 2005 7:56 pm

Seems everyone is as clueless as I am.
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canalon
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Post by canalon » Tue Jul 05, 2005 8:32 pm

You may find some help on this forum http://www.protocol-online.org/forums/index.php which is more technically oriented that this one. Good Luck

Patrick
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palpha3
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Post by palpha3 » Wed Jul 06, 2005 8:05 pm

thanks i'll try there
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