Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.
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I haven't resreached this but I'll give it a try:unlucky jester wrote:I have to list ten possible sources of procedural errors which may affect the accuracy of P.A.G.E.
Can anybody help?
accuracy of measuring equipment such as pipettes,
how similar the background colour of gel might be to those of the bands (I had that prob in a prac),
the logarithmic measurements of the distances the bands travel, ie - the nearer these are to the beginning the closer their size values are so a band could be measured as lets say 7 kb when it's really 15 kb (that happened to me in another prac),
not sure but would amount of electric current and how long it was on affect how far the bands run? I would think it would possibly.
That's all I can think of for now.
It'll be many books, a good one is principles of biochemistry but it's in tounes of others. I could explain it but just look it up and then if there's something specific you can't find ask here. Why do so many members not look in books, just post their questions straight here?achengseu wrote:do you know how the principles of gel elecrophoesis allow for DNA seperation?
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