Poly acrilamide gel electrophoresis (P.A.G.E.)

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

Moderators: honeev, Leonid, amiradm, BioTeam

Post Reply
unlucky jester
Garter
Garter
Posts: 1
Joined: Mon Jan 17, 2005 10:59 am

Poly acrilamide gel electrophoresis (P.A.G.E.)

Post by unlucky jester » Mon Jan 17, 2005 11:11 am

I have to list ten possible sources of procedural errors which may affect the accuracy of P.A.G.E.
Can anybody help?

rexarunraj
Garter
Garter
Posts: 4
Joined: Tue Feb 21, 2006 3:32 pm

Post by rexarunraj » Tue Feb 21, 2006 3:57 pm

we have lots of trouble shooting with PAGE.

List them.... I'll try...

User avatar
Sepals
Coral
Coral
Posts: 364
Joined: Sat Dec 31, 2005 12:45 am
Location: Manchester

Re: Poly acrilamide gel electrophoresis (P.A.G.E.)

Post by Sepals » Sun Feb 26, 2006 12:58 am

unlucky jester wrote:I have to list ten possible sources of procedural errors which may affect the accuracy of P.A.G.E.
Can anybody help?
I haven't resreached this but I'll give it a try:

accuracy of measuring equipment such as pipettes,

how similar the background colour of gel might be to those of the bands (I had that prob in a prac),

the logarithmic measurements of the distances the bands travel, ie - the nearer these are to the beginning the closer their size values are so a band could be measured as lets say 7 kb when it's really 15 kb (that happened to me in another prac),

not sure but would amount of electric current and how long it was on affect how far the bands run? I would think it would possibly.

That's all I can think of for now.
Image

User avatar
achengseu
Garter
Garter
Posts: 2
Joined: Sun Feb 26, 2006 9:57 pm
Location: San Diego
Contact:

Post by achengseu » Sun Feb 26, 2006 10:16 pm

do you know how the principles of gel elecrophoesis allow for DNA seperation?

User avatar
Sepals
Coral
Coral
Posts: 364
Joined: Sat Dec 31, 2005 12:45 am
Location: Manchester

Post by Sepals » Sun Mar 05, 2006 3:14 pm

achengseu wrote:do you know how the principles of gel elecrophoesis allow for DNA seperation?
It'll be many books, a good one is principles of biochemistry but it's in tounes of others. I could explain it but just look it up and then if there's something specific you can't find ask here. Why do so many members not look in books, just post their questions straight here? :?
Image

User avatar
Nithin
Garter
Garter
Posts: 49
Joined: Sun Mar 05, 2006 1:24 pm
Location: India, Asia. (Too many people!!!)
Contact:

Post by Nithin » Sun Mar 05, 2006 3:45 pm

Hi Unlucky Jester,
I am sure that that the passage of electric current would be a very important factor in PAGE. The problem arising due to insufficient contrast between the band color and gel background seems quite unlikely. Not sure...But I am sure of the electric current factor.

Post Reply

Who is online

Users browsing this forum: No registered users and 6 guests