Protein extraction from leaf tissue

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ss_kalve
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Protein extraction from leaf tissue

Post by ss_kalve » Wed Mar 18, 2009 5:42 am

Dear all,
can anybody tell me the procedure to extract protein from plant leaf tissue by sonication.actually i just want the buffer composition & frequency,temperature and duration of sonication.Further i want to proceed with 2D electrophoresis with this isolated protein.

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