Crystallization of small RNA Hairpin Structures

Discussion of all aspects of cellular structure, physiology and communication.

Moderators: honeev, Leonid, amiradm, BioTeam

Post Reply
cgh24
Garter
Garter
Posts: 2
Joined: Mon Mar 09, 2009 6:37 pm

Crystallization of small RNA Hairpin Structures

Post by cgh24 » Mon Mar 09, 2009 6:56 pm

The RNA GUAA Tetraloop attached to a short RNA helix has not been successfully crystallized. I'm trying to understand why this hasn't been possible. The only reasoning I've been able to come up with online and textbook research is that perhaps when the strands are cooling down and annealing they bond with neighboring RNA sequences instead of the sequence from their own chain, preventing the presentation of the hairpin in any crystals that form. Am I overlooking anything from the crystallization process of RNA, or does anyone else have any ideas?


ex. of GUAA Tetraloop is attached
Attachments
Picture 2.png
(2.95 KiB) Downloaded 1310 times

Post Reply

Who is online

Users browsing this forum: No registered users and 8 guests