miRNA isolation from total RNA

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Pennlab
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miRNA isolation from total RNA

Post by Pennlab » Mon Mar 09, 2009 3:15 pm

I isolated total RNA from mouse lung tissues by using Qiagen RNeasy minikit( Triazol-chloroform-spincolumn). Total RNA was very good in quality and quantity when I checked with Agilent. Now I want to isolate miRNA from those total RNA. I already tried with Ambion kit. But it did not work. Now one thing is in my mind whether I lost miRNA when I isolated total RNA. Do you have any suggesstion about isolating miRNA from those sample?

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