Linkage Map for a Plasmid?

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Mithril
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Linkage Map for a Plasmid?

Post by Mithril » Thu Feb 05, 2009 1:41 am

I've been assigned an old AP free response question for homework and need a little help starting. I have to give an actual example of a plasmid linkage map done with restriction enzymes and explain how the map is assembled. I'm not sure I understand the question entirely (I've asked for clarification twice and it hasn't helped). Why would we be expected to know an actual of an example of a plasmid linkage map? I looked up linkage map in the textbook and it only talked about linkage maps that are made by recombination frequencies, which obviously isn't what the question is asking for.
If someone could at least give me a hint about what to put into a search engine, I'd be really grateful.

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Post by Mithril » Sat Feb 07, 2009 7:42 pm

Is what I'm looking for a restriction map? and what is agarose gel?

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Post by Mithril » Sat Feb 07, 2009 8:17 pm

I *think* this website has what I'm looking for, but I don't really understand what it means. What is a vector in this context? Is vector the plasmid itself? I also don't really understand their reasoning with the Kpn I cutting the 3000 base pair fragment into two. What do they mean by the "big" fragment?
http://www.vivo.colostate.edu/hbooks/ge ... /maps.html

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Post by canalon » Sun Feb 08, 2009 1:05 am

Agarose gel, see here: http://en.wikipedia.org/wiki/Gel_electrophoresis
The webpage seems a good description of the creation of a restriction map. In the 4 paragraph the studied construction is described. In this case the plasmid carrying the insert is indeed the vector. And they have a "big" fragment because in this case the gel is not accurate enough to give a good estimate of the size hence just big.
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)

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Post by Mithril » Sun Feb 08, 2009 5:12 pm

Ok, thanks. What I mainly don't understand now is what they mean by vector. Is the vector the known part of the plasmid?

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Post by Mithril » Sun Feb 08, 2009 5:19 pm

I'm also confused about the difference between a restriction map and a linkage map. I'm confused on how you can get a linkage map with restriction enzymes because I understood that a linkage map is created using crossing over frequencies, which I don't think requires enzymes.

I'm pretty discouraged right now because I think I've done the entire assignment wrong and it's due tomorrow...

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Post by Mithril » Sun Feb 08, 2009 7:18 pm

Am I right in thinking that the circular map shown here is a linkage map? http://www.nature.com/ismej/journal/v2/ ... 7105a.html

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Re:

Post by Mithril » Sun Feb 08, 2009 7:21 pm

Mithril wrote:Am I right in thinking that the circular map shown here is a linkage map? http://www.nature.com/ismej/journal/v2/ ... 7105a.html


If not, how can I find a linkage map using restriction enzymes? I'm completely stumped here...

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